ABSTRACT
To determine the percentage of false positive testing for transfusion transmitted infections [TTIs] using immunochromatographic test [ICT] as first line of screening tests and its effect on loss of volunteer blood donors. Over a period of three months, samples from blood bags of donors undergoing phlebotomy at teaching hospital blood banks in Lahore were screened for human immunodeficiency virus [HIV], hepatitis B [HBV] and hepatitis C [HCV] by immunochromatographic tests. Those found positive on initial screening were re-tested by ELISA method at the screening laboratory of the Institute of Haematology and Blood Transfusion Service, Punjab. Lahore. Out of a total of 62090 voluntary blood donors, 469 donors were found to be initially reactive for either HIV, HBV or HCV. Amongst these 96 [0.15%] blood donors were found to have tested falsely positive for HIV, HBV or HCV as compared to testing by ELISA. False positive testing rate of 0.15% or 96 out of a total of 62090 donors is rather small in terms of loss of voluntary donors and appropriate utilization of available resources. Although immunochromatographic testing is not the gold standard, however it serves an important purpose of initial donor screening
Subject(s)
Humans , Multiphasic Screening/methods , Multiphasic Screening/standards , Multiphasic Screening/statistics & numerical data , Blood Donors/psychology , False Positive Reactions , Phlebotomy/statistics & numerical data , HIV , Blood Transfusion/adverse effects , Hepatitis B/diagnosis , Hepatitis C/diagnosis , Enzyme-Linked Immunosorbent Assay/statistics & numerical dataABSTRACT
Os autores analisam os metodos diagnosticos para infeccao urinaria (IU) na crianca, suas vantagens e deficiencias relativas. Discutem as indicacoes dos metodos segundo tipo de paciente e recursos disponiveis.